| Résumé : |
Objectives: The aim of this study was to investigate the effects of propofol on the activation of hippocampal calcium/calmodulin-dependent protein kinase IIa (CaMKIIa) in electroconvulsive therapy (ECT) in a rat model of depression.Methods: Sprague-Dawley rats were stressed repeatedly for 28 days to establish a depressed model. Forty depressed rats were then randomly assigned (n = 10 per group) to the depression group, propofol group (received propofol once a day for 1 week), ECT group (treated with ECT once a day for 1 week), or propofol + ECT group (treated with ECT pretreated and propofol once a day for 1 week). Their depressive state was assessed using the sucrose preference test and open-field test, whereas their learning and memory were evaluated using the Morris water maze task. The expression levels of CaMKIIa and phosphorylated CaMKIIa (pCaMKIIa) were detected by immunohistochemistry.Results: Compared with the depression group, the ECT and propofol + ECT groups had higher sucrose preference percentages and scored higher on the open-field test. The ECT group exhibited longer escape latency, shorter space exploration time, down-regulated expressions of CaMKIIa and pCaMKIIa in the hippocampus, and lower pCaMKIIa/CaMKIIa values. The propofol + ECT group showed up-regulated expressions of CaMKIIa and pCaMKIIa in the hippocampus. Compared with the ECT group, the propofol + ECT group exhibited shorter escape latency, longer space exploration time, up-regulated expressions of CaMKIIa and pCaMKIIa, and higher pCaMKIIa/CaMKIIa values.Conclusions: Propofol may potentially alleviate ECT-induced learning/memory impairment in depressed rats by enhancing CaMKIIa activation in the hippocampus. |
Ajouter le résultat dans votre panier Faire une suggestion Affiner la recherche Interroger des sources externesEffects of Propofol on the Activation of Hippocampal CaMKIIa in Depressed Rats Receiving Electroconvulsive Therapy in The Journal of ECT, Vol 28-N°4 December (2012)
